| 355 | 0 | 104 |
| 下载次数 | 被引频次 | 阅读次数 |
针对可吸收人工韧带生物功能化程度低的难题，通过异形纺丝技术与后加工工艺调控，获得可连续制备、比表面积高的六叶形聚乳酸纤维，编织后获得力学强度高于天然组织的异形纤维基人工韧带。对以上纤维材料的力学性能、形貌结构和细胞相容性展开研究。结果表明，通过对纺丝和牵伸工艺的调控与优化，获得“类六芒星形”截面且力学强度高达3.28 cN/dtex的六叶形聚乳酸纤维；以该纤维为基本单元编织人工韧带，所得人工韧带的断裂强度(56.5 MPa)远高于人体前交叉韧带(37.8 MPa),且具有更密集的沟槽拓扑结构。细胞试验结果表明，截面异形化的PLA人工韧带，得益于其更大的比表面积，显著促进了成纤维细胞的黏附、增殖等。研究可为开发新型可吸收、促修复人工韧带提供理论依据和实践参考。
Abstract：In response to the challenge of low biological functionalization in absorbable artificial ligaments, hexagonal polylactic acid(PLA) fibers with high specific surface area were developed through irregular spinning technology and post-processing optimization. The fibers were woven into artificial ligaments exhibiting higher mechanical strength than that of natural tissues. The research investigated the mechanical properties, morphological characteristics, and cellular compatibility of these fiber materials. Test results show that precise control of spinning and drawing parameters enables the successful fabrication of PLA fibers with a distinctive “quasi hexagonal star shaped” cross-section, achieving an exceptional mechanical strength of 3.28 cN/dtex. When woven into artificial ligaments, these fibers maintain significantly higher fracture strength(56.5 MPa) than that(37.8 MPa) of natural human anterior cruciate ligaments while presenting a more intricate groove topology. Cellular studies reveal that the unique shaped PLA artificial ligaments, owing to their enhanced specific surface area, markedly improves fibroblast adhesion, proliferation, and other cellular activities. This investigation provides both theoretical foundations and practical insights for advancing the development of novel absorbable and regenerative artificial ligaments.
[1] NO Y J,CASTILHO M,RAMASWAMY Y,et al.Role of biomaterials and controlled architecture on tendon/ligament repair and regeneration [J].Advanced Materials,2020,32(18):e1904511.
[2] 闫红芹，谢飞.PLA纤维的基本力学性能研究[J].化工新型材料，2015,43(3):143-145.YAN H Q,XIE F.Study on basic mechanical property of PLA fiber [J].New Chemical Materials,2015,43(3):143-145.
[3] LAURENCIN C T,FREEMAN J W.Ligament tissue engineering:an evolutionary materials science approach [J].Biomaterials,2005,26(36):7530-7536.
[4] COOPER J A,LU H H,KO F K,et al.Fiber-based tissue-engineered scaffold for ligament replacement:design considerations and in vitro evaluation [J].Biomaterials,2005,26(13):1523-1532.
[5] LU H H,COOPER J A,Jr.,MANUEL S,et al.Anterior cruciate ligament regeneration using braided biodegradable scaffolds:in vitro optimization studies [J].Biomaterials,2005,26(23):4805-4816.
[6] COOPER J A,Jr.,SAHOTA J S,GORUM W J,et al.Biomimetic tissue-engineered anterior cruciate ligament replacement [J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(9):3049-3054.
[7] FLEMMING R G,MURPHY C J,ABRAMS G A,et al.Effects of synthetic micro- and nano-structured surfaces on cell behavior [J].Biomaterials,1999,20(6):573-588.
[8] WANG X,DING B,LI B.Biomimetic electrospun nanofibrous structures for tissue engineering [J].Mater Today (Kidlington),2013,16(6):229-241.
[9] KAISER J P,REINMANN A,BRUININK A.The effect of topographic characteristics on cell migration velocity [J].Biomaterials,2006,27(30):5230-5241.
[10] LIU W,THOMMPOULOS S,XIA Y.Electrospun nanofibers for regenerative medicine [J].Advanced Healthcare Materials,2012,1(1):10-25.
[11] YEOM B Y,POURDEYHIMI B.Web fabrication and characterization of unique winged shaped,area-enhanced fibers via a bicomponent spunbond process [J].Journal of Materials Science,2011,46(10):3252-3257.
[12] DONG Q,CAI J,WANG H,et al.Artificial ligament made from silk protein/Laponite hybrid fibers [J].Acta Biomaterialia,2020,106:102-113.
[13] ALTMAN G H,HRAN R L,LU H H,et al.Silk matrix for tissue engineered anterior cruciate ligaments [J].Biomaterials,2002,23(20):4131-4141.
[14] LI X,SNEDEKER J G.Wired silk architectures provide a biomimetic ACL tissue engineering scaffold [J].Journal of the Mechanical Behavior of Biomedical Materials,2013,22:30-40.
[15] TABI T,HAJBA S,KOVACS J G.Effect of crystalline forms (α′ and α) of poly(lactic acid) on its mechanical,thermo-mechanical,heat deflection temperature and creep properties [J].European Polymer Journal,2016,82:232-243.
[16] BATTEGAZZORE D,BOCCHINI S,FRACHE A.Crystallization kinetics of poly(lactic acid)-talc composites [J].Express Polymer Letters,2011,5(10):849-858.
[17] 赵琪.异形喷丝板的孔形设计方法[J].合成纤维工业，1988,11(4):22-27.ZHAO Q.Design method of hole shape for irregular spinneret [J].China Synthetic Fiber Industry,1988,11(4):22-27.
[18] 李慧遥.基于透明尼龙的反光纤维的制备[D].上海：东华大学，2014:42.LI H Y.Preparation of reflective fiber based on transparent nylon [D].Shanghai:Donghua University,2014:42.
[19] RADJBIAN M,KISH M H,MOHAMMADI N,et al.Characterization of poly(lactic acid) multifilament yarns.I.the structure and thermal behavior [J].Journal of Applied Polymer Science,2010,117:1516-1525.
[20] BRENNAN D A,CONTE A A,KANSKI G,et al.Mechanical considerations for electrospun nanofibers in tendon and ligament repair [J].Advanced Healthcare Materials,2018,7(12):1701277.
[21] PARK S J,LEE B K,NA M H,et al.Melt-spun shaped fibers with enhanced surface effects:fiber fabrication,characterization and application to woven scaffolds [J].Acta Biomaterialia,2013,9(8):7719-7726.
[22] EMONTS C,BAUER B,BUCHTER C,et al.Effect of collagen coating and fiber profile on tenocyte growth on braided poly-ε-caprolactone scaffolds for tendon and ligament regeneration [J].International Journal of Molecular Sciences,2025,26:1735.
[23] TAI C,HUANG C,CHOU B,et al.Profiled polyethylene terephthalate filaments that incorporate collagen and calcium phosphate enhance ligamentisation and bone formation [J].European Cells & Materials,2022,43:252-266.
[24] SCHELLENBERG A,ROSS R,ABAGNALE G,et al.3D non-woven polyvinylidene fluoride scaffolds:fibre cross section and texturizing patterns have impact on growth of mesenchymal stromal cells [J].Plos One,2014,9 (4):e94353.
[25] CHUNG S,GAMCSIK M P,KING M W.Novel scaffold design with multi-grooved PLA fibers [J].Biomedical Materials,2011,6(4):045001.
基本信息:
DOI：10.19886/j.cnki.dhdz.2025.0192
中图分类号:TS106.67
引用信息:
[1]陆腱,俞森龙,吴琪琳,等.聚乳酸异形纤维基人工韧带的制备及性能[J].东华大学学报(自然科学版),2025,51(06):184-191.DOI:10.19886/j.cnki.dhdz.2025.0192.
基金信息:
国家重点研发计划(2021YFA1201300); 国家自然科学基金(52303179)