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从力学性能和组成成分两方面还原乳腺肿瘤细胞的生长环境，开发了一种负载富血小板血浆的纤维-水凝胶复合结构支架。通过检测支架的元素组成和化学结构，确认支架中各组分的成功负载，并利用扫描电镜、溶胀测试和水接触角测试表征了支架的表面形貌和理化性能。研究表明：复合支架具有适用于物质传输的孔隙和利于细胞黏附的表面性能；加入纤维显著提高了水凝胶的力学性能，且复合支架具有与乳腺肿瘤组织接近的弹性模量((4.79±0.45)kPa);与二维(2D)培养和无纤维的水凝胶支架相比，复合支架上培养的乳腺肿瘤细胞增殖能力提高了33.1%,显示出细胞聚集成球的特性，并对化疗药物显示出更低的敏感性。复合支架有助于肿瘤学体外研究和预测抗肿瘤药物疗效。
Abstract：A fiber-hydrogel composite scaffold loaded with platelet-rich plasma was developed to simulate the microenvironment of breast tumor using mechanical and composition cues. The elemental composition and chemical structure were tested to confirm the successful loading of each component in the scaffold. The surface morphology and physicochemical properties of the scaffolds were characterized using scanning electron microscopy, swelling test, and water contact angle test. The study demonstrates that: the composite scaffold has appropriate pores for mass transfer as well as surface characteristics for cell adhesion. The fibers significantly improve the mechanical properties of the hydrogel, and the composite scaffold has similar mechanical properties to the breast tumor((4.79±0.45)kPa). Tumor cells cultured within this scaffold have higher proliferation capacities than 2D culture by a 33.1% improvement. The cells in the composite scaffold show the ability to assemble into spheres, and less sensitive to chemotherapy when compared with 2D culture and fiber-free. It is anticipated that this composite scaffold will be helpful for in vitro oncology studies and antitumor drug efficacy prediction.
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基本信息:
DOI：10.19886/j.cnki.dhdz.2022.0361
中图分类号:R318.08;TB332
引用信息:
[1]傅思佳,刘星星,胡梦博等.用于乳腺肿瘤细胞三维培养的纤维-水凝胶复合支架的制备及表征[J],2024,50(01):11-19.DOI:10.19886/j.cnki.dhdz.2022.0361.
基金信息:
国家自然科学基金青年科学基金项目(81802569)