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当NO~-3—N和Cr(Ⅵ)共存时，电子供体是影响其处理效果的关键因素。本研究以黄铁矿、葡萄糖以及黄铁矿+葡萄糖为电子供体构建自养、异养以及混养颗粒污泥系统，探究黄铁矿混养系统处理NO~-3—N和Cr(Ⅵ)的优势及内在机制。结果表明，随着Cr(Ⅵ)质量浓度增加至6 mg/L,异养和混养体系呈现更好的脱氮除铬能力；然而，在COD质量浓度与N元素质量浓度的比值(C/N)比较低的条件下，混养体系比异养系统呈现更好的处理能力。微生物分析表明EPS中微生物副产物和酪氨酸类物质减少，色氨酸显著增加，Zeta电位降低，增强了污泥对Cr(Ⅵ)的抗冲击性；而自养菌Thiobacillus(1.65%～2.81%)与异养菌Denitratisoma(2.57%～8.70%)/Aegiribacteria(3.65%～8.43%)等菌属的富集与共存，解释了黄铁矿混养体系良好效果的原因。研究结果对NO~-3—N和Cr(Ⅵ)复合污染的高效低碳处理提供新策略。
Abstract：When NO~-3—N and Cr(Ⅵ) co-exist, the electron donor is a key factor affecting their treatment effectiveness. In this study, we constructed autotrophic, heterotrophic, and mixotrophic granular sludge systems using pyrite, glucose, and pyrite+glucose as electron donors to investigate the advantages and intrinsic mechanisms of the pyrite mixotrophic system for treating NO~-3—N and Cr(Ⅵ). The results show that the heterotrophic and mixotrophic systems present better nitrogen and chromium removal capacities as the Cr(Ⅵ) concentration increases to 6 mg/L; however, the mixotrophic system presents better treatment capacity than the heterotrophic system under the conditions where the ratio of COD mass concentration to elemental N mass concentration(C/N) is relatively low conditions. Microbiological analyses showed a decrease in microbial by-products and tyrosine, a significant increase in tryptophan, and a decrease in zeta potential in EPS, which enhanced the sludge impact resistance to Cr(Ⅵ); whereas, autotrophic bacterial genus Thiobacillus(1.65%-2.81%) and heterotrophic bacterium Denitratisoma(2.57%-8.70%)/Aegiribacteria(3.65%-8.43%) and other genera of bacteria were enriched and coexisted, explaining the good results of the pyrite mixotrophic system. The results of the study provide a new strategy for efficient and low-carbon treatment of NO~-3—N and Cr(Ⅵ) composite pollution.
[1] ZAINUDIN A M,ISA N M,HUSIN N H,et al.Groundwater potability assessment through integration of pollution index of groundwater (PIG) and groundwater quality index (GWQI) in Linggi River Basin,Negeri Sembilan,Malaysia[J].Groundwater for Sustainable Development,2024,26:101225.
[2] HAUSLADEN D M,ALEXANDER-OZINSKAS A,MCCLAIN C,et al.Hexavalent chromium sources and distribution in California groundwater[J].Environmental Science & Technology,2018,52(15):8242-8251.
[3] FU X R,HOU R R,YANG P,et al.Application of external carbon source in heterotrophic denitrification of domestic sewage:a review[J].Science of The Total Environment,2022,817:153061.
[4] LONG M,ZHOU C,XIA S Q,et al.Concomitant Cr(VI) reduction and Cr(III) precipitation with nitrate in a methane/oxygen-based membrane biofilm reactor[J].Chemical Engineering Journal,2017,315:58-66.
[5] MAO J,CHE H J,WANG X Y,et al.Mixotrophic denitrification of glucose polymer-based pyrite tailings for enhanced nitrogen and phosphorus removal of municipal tailwater[J].Water,2022,14(12):1868.
[6] WANG Q,ZHAO Y X,ZHANG C G,et al.New insights into hexavalent chromium exposure in electron donor limited denitrification:bio-electron behavior[J].Bioresource Technology,2023,380:129088.
[7] SUN X Y,LI X P,TANG S,et al.A review on algal-bacterial symbiosis system for aquaculture tail water treatment[J].Science of The Total Environment,2022,847:157620.
[8] LIU T,HU Y T,CHEN N,et al.High redox potential promotes oxidation of pyrite under neutral conditions:Implications for optimizing pyrite autotrophic denitrification[J].Journal of Hazardous Materials,2021,416:125844.
[9] LÜ Y,LI Z F,LI J F,et al.Synergetic effect of pyrite on Cr(VI) removal by zero valent iron in column experiments:an investigation of mechanisms[J].Chemical Engineering Journal,2018,349:522-529.
[10] LUO X X,SU J F,SHAO P H,et al.Efficient autotrophic denitrification performance through integrating the bio-oxidation of Fe(II) and Mn(II)[J].Chemical Engineering Journal,2018,348:669-677.
[11] LEIRO J A,MATTILA S S,LAAJALEHTO K.XPS study of the sulphur 2p spectra of pyrite[J].Surface Science,2003,547(1/2):157-161.
[12] BAI Y,WANG Z Z,LENS P N L,et al.Role of iron(II) sulfide in autotrophic denitrification under tetracycline stress:Substrate and detoxification effect[J].Science of The Total Environment,2022,850:158039.
[13] 李亚楠，张皓，王宇晖，等.无机-有机基质配置对反硝化生物滤池脱氮除磷效能的影响机制[J].东华大学学报(自然科学版),2023,49(2):150-158.LI Y N,ZHANG H,WANG Y H,et al.The influence mechanism of nitrogen and phosphorus removal efficiency in a denitrification biofilter with inorganic and organic matter substrate[J].Journal of Donghua University (Natural Science),2023,49(2):150-158.
[14] SUN F L,FAN L L,WANG Y S,et al.Metagenomic analysis of the inhibitory effect of chromium on microbial communities and removal efficiency in A2O sludge[J].Journal of Hazardous Materials,2019,368:523-529.
[15] XU R,SUN X X,HÄGGBLOM M M,et al.Metabolic potentials of members of the class Acidobacteriia in metal-contaminated soils revealed by metagenomic analysis[J].Environmental Microbiology,2022,24(2):803-818.
[16] SHI S H,HE X J,HE L,et al.Overlooked pathways of endogenous simultaneous nitrification and denitrification in anaerobic/aerobic/anoxic sequencing batch reactors with organic supplementation[J].Water Research,2023,230:119493.
[17] WANG W T,CHEN C M,HUANG X P,et al.Chromium(VI) adsorption and reduction in soils under anoxic conditions:the relative roles of iron (oxyhr)oxides,iron(II),organic matters,and microbes[J].Environmental Science & Technology,2024,58(41):18391-18403.
[18] ZHAO L F,XUE L Y,WANG L,et al.Simultaneous heterotrophic and FeS2-based ferrous autotrophic denitrification process for low-C/N ratio wastewater treatment:Nitrate removal performance and microbial community analysis[J].Science of The Total Environment,2022,829:154682.
[19] ZHANG H G,XU Z S,ZHOU P P,et al.Simultaneous nitrate and chromium removal mechanism in a pyrite-involved mixotrophic biofilter[J].Environmental Science and Pollution Research International,2023,30(59):123882-123892.
[20] MA R H,SHI Y Q,CHEN Y C.The alleviation of Cr(Ⅵ) stress on simultaneous nitrification and denitrification process of Acinetobacter haemolyticus RH19[J].Water Research,2025,273:122968.
[21] WANG Q,ZHAO Y X,LIU Y N,et al.Recovery mechanism of bio-promoters on Cr(VI) suppressed denitrification:Toxicity remediation and enhanced electron transmission[J].Water Research,2024,252:121230.
[22] WU M R,XU Y N,ZHAO C X,et al.Efficient nitrate and Cr(VI) removal by denitrifier:the mechanism of S.oneidensis MR-1 promoting electron production,transportation and consumption[J].Journal of Hazardous Materials,2024,469:133675.
[23] ZHANG Z H,HUANG Z A,LI H X,et al.Impact of nitrate on the removal of pollutants from water in reducing gas-based membrane biofilm reactors:a review[J].Membranes,2024,14(5):109.
[24] WANG Q,ZHANG C G,SONG J X,et al.Inhibitory mechanism of Cr(VI) on sulfur-based denitrification:bio-toxicity,bio-electron characteristics,and microbial evolution[J].Journal of Hazardous Materials,2024,472:134447.
[25] CHEN W,WESTERHOFF P,LEENHEER J A,et al.Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter[J].Environmental Science & Technology,2003,37(24):5701-5710.
[26] LUO X X,ZHOU X Y,PENG C Y,et al.Bioreduction performance of Cr(VI) by microbial extracellular polymeric substances (EPS) and the overlooked role of tryptophan[J].Journal of Hazardous Materials,2022,433:128822.
[27] NKOH J N,YAN J,HONG Z N,et al.Effects of citrate,oxalate,and phosphate on the sorption of Cr(VI) by extracellular polymeric substances[J].Journal of Water Process Engineering,2020,37:101510.
[28] LI Z L,LU P L,ZHANG D J,et al.Population balance modeling of activated sludge flocculation:Investigating the influence of Extracellular Polymeric Substances (EPS) content and zeta potential on flocculation dynamics[J].Separation and Purification Technology,2016,162:91-100.
[29] LIU X Y,LIU J,DENG D K,et al.Investigation of extracellular polymeric substances (EPS) in four types of sludge:Factors influencing EPS properties and sludge granulation[J].Journal of Water Process Engineering,2021,40:101924.
[30] CAO G,GAO J Z,SONG J X,et al.Performance and mechanism of chromium reduction in denitrification biofilm system with different carbon sources[J].Science of The Total Environment,2024,906:167191.
[31] XIAO W D,ZHANG Q,ZHAO S P,et al.Citric acid secretion from rice roots contributes to reduction and immobilization of Cr(VI) by driving microbial sulfur and iron cycle in paddy soil[J].Science of The Total Environment,2023,854:158832.
[32] LI B Q,XU R,SUN X X,et al.Microbiome-environment interactions in antimony-contaminated rice paddies and the correlation of core microbiome with arsenic and antimony contamination[J].Chemosphere,2021,263:128227.
[33] WANG C Y,ZHU B,GUO Y T,et al.Assessment of the pollution of soil heavy metal(loid)s and its relation with soil microorganisms in wetland soils[J].Sustainability,2022,14(19):12164.
[34] WU Y C,ZAIDEN N,CAO B.The core- and pan-genomic analyses of the genus Comamonas:from environmental adaptation to potential virulence[J].Frontiers in Microbiology,2018,9:3096.
基本信息:
DOI：10.19886/j.cnki.dhdz.2025.0010
中图分类号:X523
引用信息:
[1]郭靖,周盼盼,王宇晖,等.黄铁矿混养系统对硝酸盐和Cr(Ⅵ)的去除机制研究[J].东华大学学报(自然科学版),2026,52(01):31-39.DOI:10.19886/j.cnki.dhdz.2025.0010.
基金信息:
中央高校基本科研业务费(24D111311); 国家自然科学基金(52170152)
2026-02-15
2026-02-15